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In the present study, an alternative method, Sebia A1c Pro (Sebia, Bedford, MA, USA) was validated and compared with the reference method HPLC in a real-life situation. The HbA1c results were obtained with the Sebia A1c Pro and the reference HPLC method. The laboratory director validated the results obtained with the two methods before sending them to the medical director for calculating the correlation coefficient. The correlation coefficient was 0.977 (n = 115, p < 0.001). The method imprecision was within limits (CV, 2-3.7%), and the method bias was not significant. The study proved that the Sebia A1c Pro is a reliable and user friendly method for HbA1c analysis.
The results obtained from the ICEB method were similar to the HPLC method in clinical conditions. In addition, the method was feasible for large-scale studies as it requires relatively smaller quantities of blood and is more user friendly for the laboratory staff. This method can be used in clinical practice as an alternative to the reference method as it is cost effective and requires minimal expertise. It can also be used as a reference method for clinical trials as the reference values obtained for HbA1c are similar to those obtained from the DCCT.
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